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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Effect of AdpA deficiency on S. griseus cells grown in SMM medium

ABSTRACT: Comparative hybridization of wild type versus AdpA deficient mutant grown on SMM medium 3 x dual channel slides, dye swap

ORGANISM(S): Streptomyces griseus subsp. griseus NBRC 13350

SUBMITTER: Genki Akanuma

PROVIDER: E-GEOD-14770 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Dynamic changes in the extracellular proteome caused by absence of a pleiotropic regulator AdpA in Streptomyces griseus.

Akanuma Genki G Hara Hirofumi H Ohnishi Yasuo Y Horinouchi Sueharu S

Molecular microbiology 20090804 5

In Streptomyces griseus, A-factor (2-isocapryloyl-3R-hydroxymethyl-gamma-butyrolactone) triggers morphological development and secondary metabolism by inducing a pleiotropic transcriptional regulator AdpA. Extracellular proteome analysis of the wild-type and DeltaadpA strains grown to the end of the exponential phase in liquid minimal medium revealed that 38 secreted proteins, including many catabolic enzymes, such as protease, glycosyl hydrolase and esterase, were produced in an AdpA-dependent ...[more]

PMID: 19678896

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