Transcription profiling of mouse embryo at E16.5 and E18.5, to monitor changes in gene expression related to maturation of the heart
Ontology highlight
ABSTRACT: Timed matings were performed using C57BL/6 mice and pregnant females sacrificed starting at embryonic day (E) 10.5, and then in daily intervals until E14.5. Gene expression was be further analyzed at E16.5 and E18.5, to monitor changes in gene expression related to maturation of the heart. At stage 10.5, the rostral and caudal parts of the embryo were removed and the middle part, which includes the heart, was subjected to expression analysis. From embryonic day 11.5 on, we isolated embryonic hearts and separated the ventricular from the atrial chambers.
Project description:Timed matings were performed using C57BL/6 mice and pregnant females sacrificed starting at embryonic day (E) 10.5, and then in daily intervals until E14.5. Gene expression was be further analyzed at E16.5 and E18.5, to monitor changes in gene expression related to maturation of the heart. At stage 10.5, the rostral and caudal parts of the embryo were removed and the middle part, which includes the heart, was subjected to expression analysis. From embryonic day 11.5 on, we isolated embryonic hearts and separated the ventricular from the atrial chambers. Keywords = heart Keywords = development Keywords = cardiac Keywords: time-course
Project description:The mammalian neocortex develops layer organizations with regional differences represented by expression of multiple genes at embryonic stages. These genes could play important roles in the formation of areal cyto-architecture, yet, the number of genes identified so far is not sufficient to explain such intricate processes. Here we collected five regions; the medial, dorsal, lateral, rostral, and occipital, from the dissected E16.5 mouse cerebral cortex and performed extensive gene expression analysis using the Affymetrix U74Av2 array. Keywords = mouse Keywords = cortex Keywords = development Keywords = regional Keywords = gene expression Keywords = medial Keywords = dorsal Keywords = lateral Keywords = rostral Keywords = occipital
Project description:We generated mouse heart organoids from mouse embryonic stem cells. The heart organoids showed both atrium-like and ventricle-like morphology similar to those of embryonic hearts. Therefore we performed RNA-seq analysis to compare atrial and ventricular gene expression profiles between mouse embryonic hearts and induced heart organoids.
Project description:Genomic microarray analysis of adrenergic-deficient (Dbh-/-) vs. wild-type control (Dbh+/+) mouse heart expression at embryonic day 10.5 (E10.5).
Project description:This dataset covers the development of 7 organs (brain, cerebellum, heart, kidney, liver, ovary and testis) from embryonic day 10.5 to adulthood.
Project description:miRNA-seq on embryonic 10.5 day mouse heart For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODE_Data_Use_Policy_for_External_Users_03-07-14.pdf
Project description:Control ChIP-seq on embryonic 10.5 day mouse heart For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODE_Data_Use_Policy_for_External_Users_03-07-14.pdf
Project description:H3K9me3 ChIP-seq on embryonic 10.5 day mouse heart For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODE_Data_Use_Policy_for_External_Users_03-07-14.pdf
Project description:H3K36me3 ChIP-seq on embryonic 10.5 day mouse heart For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODE_Data_Use_Policy_for_External_Users_03-07-14.pdf
Project description:Nanostring miRNA Array on embryonic 10.5 day mouse heart For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODE_Data_Use_Policy_for_External_Users_03-07-14.pdf