Madin Darby Canine Kidney (MDCK) Cells: Early & Late Passage

ABSTRACT: The paracellular passage of ions in epithelial systems is dictated by the repertoire of tight junction (TJ) proteins, the number and architecture of apicolateral TJ strands, and the interaction of TJ proteins with the cytoskeleton. To investigate the relative contribution of these factors and to explore their regulation, we examined a passage number dependent phenotypic change in Madin Darby Canine Kidney (MDCK) cells iteratively passaged at low density in which dilution potentials of the monolayer abruptly decreased but transepithelial resistance (TER) progressively increased. Here, we report that the phenotypic transition involved a decrease surface expression of peanut agglutinin (PNA), a decrease in steady state levels of claudin-1 and a slight decrease in E-cadherin expression in th

ORGANISM(S): Canis lupus familiaris

SUBMITTER: Virginie Aris 

PROVIDER: E-GEOD-14837 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress