LNCaP EMETINE TIME COURSE

ABSTRACT: LNCaP prostate cancer cells were treated with and without 100?g/ml Emetine Dihydrochloride Hydrate (Fluka, Buchs, Swizerland). Both Emetine-treated and untreated control cells were then incubated at 37°C for 10 hours. After the incubation, the first time point (0 min) was harvested for both treated and untreated cells. Simultaneously, Actinomycin D (Sigma-Aldrich, St Louis, MO) with the final concentration of 5ug/ml, was added to the remaining treated and untreated cells to stop new transcription. Time points of 10min, 30min, 1h, 2h, and 4h were harvested in both groups for most of the cell lines. Cell pellets were snap-frozen and mRNA extracted by using FastTrack kit (Invitrogen) according to the manufacturer’s instructions. For each time point, the untreated sample was hybridized aga

ORGANISM(S): Homo sapiens

SUBMITTER: Jeff Kiefer 

PROVIDER: E-GEOD-1488 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress