Transcription profiling by array of human hepatic cells derived from foreskin fibroblast cell line and from embryonic stem cells.

ABSTRACT: Reprogrammed somatic cells offer a valuable source of pluripotent cells that have the potential to differentiate into many cells types and provide a new tool for regenerative medicine. In the present study we differentiated induced pluripotent stem cells (iPS cells) into hepatic cells. We first showed that mouse iPS cells could from a complete liver in mouse embryo (E14.5) including hepatocytes, endothelial cells, sinusoidal cells and resident macrophages. We then designed a highly efficient hepatocyte differentiation protocol using defined factors on human embryonic stem cells (ES cells). This protocol was found to generate more than 80% albumin expressing cells that show hepatic functions and express most of liver genes as shown by microarray analyses. Similar results were obtained when

ORGANISM(S): Homo sapiens

SUBMITTER: Stephen Duncan 

PROVIDER: E-GEOD-14897 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress