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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transient Genotype-by-Environment Interactions in the Yeast Genomic Expression Response to Heat Shock

ABSTRACT: Microarray time courses followed the response of 5 yeast strains to heat shock. Expression variation due to genetic, environmental, and genotype-by-environment interactions were identified. Keywords: Timecourse and single timepoint expression studies of stress response in yeast The genomic expression response to heat shock was measured in 5 different yeast strains over the course of 2 hours. Basal expression at 25C was also compared in 4 non-lab strains to the S288c refernce. All experiments were done in duplicate, for a total of 68 Samples.

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Daniel Kvitek

PROVIDER: E-GEOD-15147 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Transient genotype-by-environment interactions following environmental shock provide a source of expression variation for essential genes.

Eng Kevin H KH Kvitek Daniel J DJ Keles Sündüz S Gasch Audrey P AP

Genetics 20091204 2

Understanding complex genotype-by-environment interactions (GEIs) is crucial for understanding phenotypic variation. An important factor often overlooked in GEI studies is time. We measured the contribution of GEIs to expression variation in four nonlaboratory Saccharomyces cerevisiae strains responding dynamically to a 25 degrees -37 degrees heat shock. GEI was a major force explaining expression variation, affecting 55% of the genes analyzed. Importantly, almost half of these expression patter ...[more]

PMID: 19966067

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