Transcription profiling of human HPV-immortalized keratinocytes

ABSTRACT: To identify early processes in carcinogenesis, we used an in vitro model, based on the initiating event in cervical cancer, human papillomavirus (HPV) transformation of keratinocytes. We compared gene expression in primary keratinocytes (K) and HPV16-transformed keratinocytes from early (E) and late (L) passages, and from benzo[a]pyrene treated L cells (BP). The transformed cells exhibit similar transcriptional changes to clinical cervical carcinoma. We revealed a contraction in expression of the apoptotic network during HF1 cell transformation, which affected the ability of L and BP cells to execute apoptosis, but did not lead to resistance to apoptotic stimuli. The contraction in the apoptotic machinery during the process of transformation was accompanied by a switch from apoptosis to ne

ORGANISM(S): Homo sapiens

SUBMITTER: Alexander Levitzki 

PROVIDER: E-GEOD-15156 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress