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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Histone H2B K111A

ABSTRACT: Total RNA from three replicate cultures of wild-type and mutant strains was isolated and the expression profiles were determined using Affymetrix arrays. Comparisons between the sample groups allow the identification of genes regulated by histone H2B K111A mutant. Comparison of genes whose mRNA levels are affected in a histone H2B K111A mutant yeast strain compared to wild-type yeast strain.

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: McKenna Kyriss

PROVIDER: E-GEOD-15203 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Novel functional residues in the core domain of histone H2B regulate yeast gene expression and silencing and affect the response to DNA damage.

Kyriss McKenna N M MN Jin Yi Y Gallegos Isaura J IJ Sanford James A JA Wyrick John J JJ

Molecular and cellular biology 20100517 14

Previous studies have identified novel modifications in the core fold domain of histone H2B, but relatively little is known about the function of these putative histone modification sites. We have mutated core modifiable residues that are conserved in Saccharomyces cerevisiae histone H2B and characterized the effects of the mutants on yeast silencing, gene expression, and the DNA damage response. We identified three histone H2B core modifiable residues as functionally important. We find that mut ...[more]

PMID: 20479120

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