Isolation and Characterisation of Renal Precursor Cells Derived from Human Embryonic Stem Cells

ABSTRACT: HES4 hESC were cultured in serum media and maintained on a layer of mouse embryonic fibroblast feeder cells at a density of 6 x 104 cells/cm2. For differentiation: hESC were differentiated for a total of 14 days. Differentiation was induced by passaging 4 human ES cell pieces onto 12 well plates seeded with 0.67 x 10E4 cells/cm2. Cells were maintained in media containing 20% FCS for 2 days before media containing 5% FCS was used. Reduced serum media was changed every second day for the remaining 12 days ESC cells were taken at time zero and RNA was isolated (hESC_undiff). Cells differentiated by the above procedure at day 14 were isolated and RNA extracted (Diff). The remaining cells were FACs sorted with antibodies to Podocalyxin, CD24 and GCTM-2. Two populations were isolated, 1: Pos

ORGANISM(S): Homo sapiens

SUBMITTER: Gabriel Kolle 

PROVIDER: E-GEOD-15257 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress