Transcription profiling of Saccharomyces cerevisiae mutant delta-spe3 delta-fms1 after spermidine treatment

ABSTRACT: The naturally occurring polyamines putrescine, spermidine or spermine are ubiquitous in all cells. Although polyamines have prominent regulatory roles in cell division and growth, precise molecular and cellular functions are not well established in vivo. In this work we have performed a microarray experiment in a polyamine mutant (delta-spe3 delta-fms1) strain to investigate the responsiveness of yeast genes to supplementation with spermidine and spermine. Expression analysis identified genes responsive to the addition of either excess spermidine (10-5 M) or spermine (10-5 M) compared to a control culture containing 10-8 M spermidine. 247 genes were up-regulated >2-fold, and 11 genes were up-regulated more than 10-fold after spermidine addition. Functional categorization of the genes showe

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: WeiPing Chen 

PROVIDER: E-GEOD-15269 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress