Discovery of novel hypermethylated gene loci in prostate cancer using genome wide coverage CpG island microarrays

ABSTRACT: In order to identify methylation changes in prostate cancer, we performed a genome-wide analysis of DNA methylation using Agilent human CpG island arrays. We then chose specific genes to validate methylation both in the same cases as were hybridized to the array (using quantitative EpiTYPER analysis) and in an independent series of prostate cancer samples (using MethyLight quantitative methylation specific PCR). We specifically chose low grade (Gleason score 6 cases) and high grade (Gleason score 8 cases) to discover methylated genes/loci that may be involved in the progression to a higher grade of prostate cancer. We collected 20 specimens consisting of 10 Gleason 6 and 10 Gleason 8 prostate cancers, and compared these to a reference lymphocyte pool (6 age matched, healthy men) to determ

ORGANISM(S): Homo sapiens

SUBMITTER: Ken Kron 

PROVIDER: E-GEOD-15298 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress