LKR13-Kras-WT1-KO

ABSTRACT: K-ras is one of the most frequently mutated human oncogenes. However, activation of K-ras can lead to either senescence or proliferation in primary cells. The precise mechanism governing these distinct outcomes remains unclear. Here we describe a novel loss-of-function screen to assess the role of specific genes identified as potential key regulators of K-ras driven oncogenesis. Using this approach, we identify the transcription factor Wt1 as an inhibitor of senescence in primary cells expressing oncogenic K-ras. Deletion or suppression of Wt1 expression leads to senescence of primary cells expressing oncogenic K-ras under the control of the native promotor and at physiological levels, but had no effect on cells expressing wild-type K-ras. Furthermore, loss of Wt1 in lung cancer cell

ORGANISM(S): Mus musculus

SUBMITTER: E.Alejandro Sweet-Cordero 

PROVIDER: E-GEOD-15326 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress