Sox2 Levels Define Pluripotent Ground States and Reprogramming Potential

ABSTRACT: Pluripotent stem cell lines derived from embryos of different stages have distinct pluripotent ground states, but similar levels of the transcription factor Oct4. Epiblast-derived pluripotent stem cells (EpiSCs), in contrast to embryonic stem (ES) cells, cannot form chimeras. We show that EpiSCs express lower levels of the transcription factors Sox2 and Klf4 than ES cells and have limited reprogramming potential, as shown by cell fusion. Sox2 overexpression dramatically increases the reprogramming potential, chimera formation, and germline contribution of EpiSCs. Therefore, although Oct4 is essential for reprogramming, the level of Sox2 defines both the reprogramming capability and the pluripotent ground states. RNA samples to be analyzed on microarrays were prepared using Qiagen RNeasy co

ORGANISM(S): Mus musculus

SUBMITTER: Marcos Arauzo-Bravo 

PROVIDER: E-GEOD-15358 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress