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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Gene expression analysis of dendritic cells treated with small chemical compounds

ABSTRACT: Identification of newer compounds to modulate dendritic cell functions. Total RNA obtained from bone marrow-derived dendritic cells treated for 6 hours with small chemical compounds or vehicle alone in the presence or absence of lipopolysaccharide (LPS).

ORGANISM(S): Mus musculus

SUBMITTER: Hironori Matsushima

PROVIDER: E-GEOD-15360 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Identification of crassin acetate as a new immunosuppressant triggering heme oxygenase-1 expression in dendritic cells.

Matsushima Hironori H Tanaka Hiroaki H Mizumoto Norikatsu N Takashima Akira A

Blood 20090428 1

By screening 720 natural compounds in a standard 2-way allogeneic mixed leukocyte reaction assay, we identified a potent immunosuppressive capacity of crassin acetate (CRA), a coral-derived cembrane diterpenoid. CRA efficiently inhibited allogeneic mixed leukocyte reaction as well as antigen-specific activation of CD4 T cells by bone marrow-derived dendritic cells (DCs). With regard to cellular targets, CRA suppressed not only mitogen-triggered T-cell activation, but also lipopolysaccharide-indu ...[more]

PMID: 19401559

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Gene expression analysis of dendritic cells treated with small chemical compounds

Project description:Identification of newer compounds to modulate dendritic cell functions.

2011-09-19 | GSE15360 | GEO

Priming conditions alter the proteome near the PVM

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2024-02-14 | PXD027716 | Pride

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2010-10-08 | E-GEOD-19006 | biostudies-arrayexpress

Effect of LMP7 and MECL1-immunoproteasome subunits deficiency on the transcriptome of mouse bone marrow-derived dendritic cells

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2014-07-02 | E-GEOD-52616 | biostudies-arrayexpress

Effect of cholera toxin or forskolin on mouse bone marrow-derived dendritic cells

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A dominant role for the methyl-CpG-binding protein Mbd2 in controlling Th2 induction by dendritic cells [gene expression]

Project description:Dendritic cells (DCs) direct CD4+ T cell differentiation into distinct T helper (Th) subsets that are vital for protection against diverse types of infection. However, the mechanisms employed by DCs to initiate Th2 responses, which are important for immunity to helminth infection as well as being a major contributor to allergic disease, remain poorly understood. We demonstrate a crucial role for methyl-CpG-binding domain-2 (Mbd2), a protein that links DNA methylation to repressive chromatin structure, in regulating DC gene expression and ability to promote Th2 immunity against either helminths or allergens. RNAs were extracted from murine (C57BL/6) Bone marrow-derived dendritic cells (BMDC) samples. Two groups of samples (three replicates each) were processed: (1) wild type, and (2) a homozygous knock-out of the Mbd2 locus.

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