Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

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Drosophila OSS cell small RNA libraries


ABSTRACT: High-throughput sequencing of Drosophila melanogaster small RNAs from OSS cells. total RNA, ~18-26nt RNAs isolated using PAGE, ligation to adapters requires 5' monophosphate and 3' OH For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf Small RNAs were sequenced from D. melanogaster OSS cells. Raw sequences were clipped by 3' linker sequences recognition, and select clipped sequences longer than 18 nt.

ORGANISM(S): Drosophila melanogaster

SUBMITTER: Nicolas Robine 

PROVIDER: E-GEOD-15378 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

A broadly conserved pathway generates 3'UTR-directed primary piRNAs.

Robine Nicolas N   Lau Nelson C NC   Balla Sudha S   Jin Zhigang Z   Okamura Katsutomo K   Kuramochi-Miyagawa Satomi S   Blower Michael D MD   Lai Eric C EC  

Current biology : CB 20091201 24


<h4>Background</h4>Piwi-interacting RNAs (piRNAs) are approximately 24-30 nucleotide regulatory RNAs that are abundant in animal gonads and early embryos. The best-characterized piRNAs mediate a conserved pathway that restricts transposable elements, and these frequently engage a "ping-pong" amplification loop. Certain stages of mammalian testis also accumulate abundant piRNAs of unknown function, which derive from noncoding RNAs that are depleted in transposable element content and do not engag  ...[more]

Publication: 1/2

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