Transcription profiling of human embryonic stem cells reveals conditioned media from SDEC supports proliferation on type I collagen

ABSTRACT: Conditioned media from a human embryonic germ cell-derived line called SDEC was found to be supportive of human embryonic stem cell growth in the absence of feeder layers on a simple type I collagen matrix. We performed gene expression studies comparing this line to non-supportive cell lines (WI-38 and Detroit 551) to try to identify gene targets responsible for this phenomenon. We used Affymetrix microarrays to identify genes that are differentially regulated in SDEC vs. non-supportive cell lines. The goal is to determine which genes maybe be contributing to human embryonic stem cell growth in the absence of a mouse fibroblast feeder layer. Experiment Overall Design: Total RNA samples were extracted from SDEC, WI-38, and Detroit 551 (D551) cell lines to compare gene expressions. Three

ORGANISM(S): Homo sapiens

SUBMITTER: Michael Betenbaugh 

PROVIDER: E-GEOD-15400 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress