Transcription profiling of human NIH-3T3 fibroblasts stably expressing the coxsackie-adenovirus receptor (CARI?1)) cells were transduced with adenovirus containing empty vector (AdEmpty) followed by addition of vehicle (DMSO) or transduced with adenovirus encoding either full length HA-tagged PPARg2 or A/B-domain deleted HA-PPARgCDE and treated with rosiglitazoneNIH-3T3 fibroblasts stably expressing the coxsackie-adenovirus receptor (CARI?1)) cells were transduced with adenovirus containing empty vector (AdEmpty) followed by addition of vehicle (DMSO) or transduced with adenovirus encoding either full length HA-tagged PPARg2 or A/B-domain deleted HA-PPARgCDE and treated with rosiglitazone reveals the PPARg2 A/B-domain plays a gene specific role in transactivation and co-factor recruitment

ABSTRACT: We have previously shown that adenoviral expression of peroxisome proliferator-activated receptors (PPARs) leads to rapid establishment of transcriptionally active complexes and activation of target gene expression within 5-8 h following transduction. Here we have used the adenoviral delivery system combined with expression array analysis to identify novel putative PPARgamma target genes in murine fibroblasts and to determine the role of the A/B-domain in PPARgamma mediated transactivation of genomic target genes. Of the 257 genes found to be induced by PPARgamma2 expression, only 25 displayed A/B-domain dependency, i.e. significantly reduced induction in the cells expressing the truncated PPARgamma lacking the A/B-domain (PPARgammaCDE). Nine of the 25 A/B-domain dependent genes were invol

ORGANISM(S): Mus musculus

SUBMITTER: Susanne Mandrup 

PROVIDER: E-GEOD-15433 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress