Transcription profiling of mouse RAW 264.7 cells treated with the oxidant hypochlorous acid (HOCl) - dose response

ABSTRACT: Hypochlorous acid (HOCl) is a potent oxidant that is produced endogenously in mammalian tissue by phagocytes. Exogenous exposure to HOCl also can occur following inhalation of chlorine gas. HOCl has been implicated as a source of oxidative stress associated atherosclerosis and other diseases. The purpose of this study was to identify dose-dependent transitions in cellular response to hypochlorous acid (HOCl), with a focus on understanding how various cellular defense and stress dose-responses overlap. Experiment Overall Design: RAW 264.7 cells were grown to 80-90% confluency in DMEM supplemented with supplemented with 10% fetal bovine serum (FBS), 10 mM HEPES, 100 U penicillin/ml, and 100 µg streptomycin/ml. Cells were treated with 0, 0.14, 0.35, 0.7, 1.4, 2.1, 2.8 or 3.5 mM HOCl in m

ORGANISM(S): Mus musculus

SUBMITTER: Courtney Woods 

PROVIDER: E-GEOD-15457 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress