High-throughput sequencing of small RNAs in chicken somite embryos
Ontology highlight
ABSTRACT:
ORGANISM(S): Gallus gallus
SUBMITTER: Helio Pais
PROVIDER: E-GEOD-15513 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
Publications
High throughput sequencing of microRNAs in chicken somites.
Rathjen Tina T Pais Helio H Sweetman Dylan D Moulton Vincent V Munsterberg Andrea A Dalmay Tamas T
FEBS letters 20090327 9
High throughput Solexa sequencing technology was applied to identify microRNAs in somites of developing chicken embryos. We obtained 651,273 reads, from which 340,415 were mapped to the chicken genome representing 1701 distinct sequences. Eighty-five of these were known microRNAs and 42 novel miRNA candidates were identified. Accumulation of 18 of 42 sequences was confirmed by Northern blot analysis. Ten of the 18 sequences are new variants of known miRNAs and eight short RNAs are novel miRNAs. ...[more]
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