Transcription profiling of human embryonic stem cells (ESC) during enhanced differentiation into multipotential mesodermal stem cells by inhibition of TGF-b signaling by using SB

ABSTRACT: Directing differentiation of human embryonic stem cells (hESC) into specific cell types using an easy and reproducible protocol is a perquisite for the clinical use of hESC in regenerative medicine protocols. Here, we report the generation of mesodermal cells with differentiation potential to myocytes, osteoblasts, chondrocytes and adipocytes. We demonstrate that during hESC differentiation as embryoid bodies (EB), inhibition of TGF-b/Activin/Nodal signaling using SB-431542 (SB) markedly up-regulated paraxial mesodermal markers (TBX6, TBX5), early myogenic transcriptional factors (Myf5, Pax7) as well as myocyte committed markers (NCAM, CD34, Desmin, MHC (fast), alpha-smooth muscle actin, Nkx2.5, cTNT). Establishing EB outgrowth cultures (SB-OG) in the presence of SB (1 uM) led to further

ORGANISM(S): Homo sapiens

SUBMITTER: Amer Mahmood 

PROVIDER: E-GEOD-15553 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress