Transcription profiling of rat tracheal airway explant treated with cigarette smoke.

ABSTRACT: Although a number of animal model studies have addressed changes in gene expression in the parenchyma and their relationship to emphysema, much less is known about the pathogenesis of cigarette smoke-induced small airway remodeling. In this study we exposed rat tracheal explants to whole smoke for 15 minutes, and then cultured the explants in air. The airway transcriptome was evaluated using RAE 230_2 gene chips. By 2 hours after starting smoke exposure, expression levels of 502 genes were changed up or down by more than 1.5 times (p values <0.01 or less) and by 24 hours 1870 genes were significantly changed up or down. These included genes involved in anti-oxidant protection, epithelial defense and remodeling, inflammatory mediators and transcription factors, and a number of unexpected

ORGANISM(S): Rattus norvegicus

SUBMITTER: Darren Gormley 

PROVIDER: E-GEOD-15563 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress