Gene Expression Changes Induced by Bacterial Superantigen, Staphylococcal Enterotoxin B

ABSTRACT: Toxic shock syndrome (TSS) is an acute, serious systemic illness caused by bacterial superantigens (BSAg). We characterized the early molecular events underlying TSS using our HLA-DR3 transgenic mouse model and studied gene expression profiling using DNA microarrays. HLA-DR3 transgenic mice were intraperitoneally treated with either PBS or bortezomib on days -1 and 0. On day 0, each treatment group was further divided in to two groups, one received 10 μg of endotoxin-reduced highly purified SEB and the other PBS alone thus making a total of 4 groups (i.e., PBS+PBS, bortezomib+PBS, PBS+SEB, bortezomib+SEB). Three hours later, mice were euthanized by CO2 asphyxiation, spleens were immediately collected and frozen in liquid nitrogen. Once all the animals had been sacrificed, total RNA from s

ORGANISM(S): Mus musculus

SUBMITTER: Chella David 

PROVIDER: E-GEOD-15571 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress