Differential effects of TNFalpha and IL1beta on FLS global gene expression profile

ABSTRACT: TNFalpha and IL1beta play a pathogenic role in rheumatoid arthritis. Both cytokines are known to activate cytokine and metalloproteinase secretion by synovial fibroblasts. In the present study, we wanted to investigate whether TNFalpha and IL1beta displayed differential effects on cultured Fibroblast-like Synovial Cells derived from RA patients. Global gene expression analyses indicated that both cytokines induced similar genes in these cells. Fibroblast-like cells (FLS) were purified from synovial biopsies from RA patients. Briefly, minced synovial fragments were digested in 1 mg/ml hyaluronidase solution (Sigma Aldrich, St Louis, MO) for 15 minutes at 37M-BM-0C and 6 mg/ml collagenase type IV (Invitrogen, Paisley, UK) for 2 hours at 37M-BM-0C. Next, cells were washed, resuspended in high

ORGANISM(S): Homo sapiens

SUBMITTER: Bernard Lauwerys 

PROVIDER: E-GEOD-15615 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress