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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Expression data from mNSc after 48 hour of treatment with CD95L-T4

ABSTRACT: In neural stem cells, stimulation of the â??death receptorâ?? CD95 does not trigger apoptosis but resulted in increased stem cell survival and neuronal specification via activation of the Src /PI3K /AKT/mTOR signalling pathway. To further characterize CD95-dependent neural stem cell survival and differentiation we used conventional gene expression profiling combined with translation state array analysis. Mouse neural stem cells grown in neurosphere cultures were stimulated with a trimerized CD95L construct (CD95L-T4) and total as well as polysomal bound RNA was isolated 48 hours after stimulation and analysed by microarrays. CD95L-T4 treatment induced a global increase in ribosome-bound mRNA and protein translation as well as changes on genes involved in neurogenesis, protein synthesis and transcription factors. Mouse neural stem cells grown in neurosphere cultures were stimulated with a trimerized CD95L construct (CD95L-T4) and total as well as polysomal bound RNA was isolated 48 hours after stimulation and hybridized on affymetrix microarrays.

ORGANISM(S): Mus musculus

SUBMITTER: Sachin Kumar

PROVIDER: E-GEOD-15623 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Expression data from mNSc after 48 hour of treatment with CD95L-T4

Project description:In neural stem cells, stimulation of the “death receptor” CD95 does not trigger apoptosis but resulted in increased stem cell survival and neuronal specification via activation of the Src /PI3K /AKT/mTOR signalling pathway. To further characterize CD95-dependent neural stem cell survival and differentiation we used conventional gene expression profiling combined with translation state array analysis. Mouse neural stem cells grown in neurosphere cultures were stimulated with a trimerized CD95L construct (CD95L-T4) and total as well as polysomal bound RNA was isolated 48 hours after stimulation and analysed by microarrays. CD95L-T4 treatment induced a global increase in ribosome-bound mRNA and protein translation as well as changes on genes involved in neurogenesis, protein synthesis and transcription factors.

2009-09-28 | GSE15623 | GEO

Transcriptomic analysis of Bmi1 overexpression in NSPC.

Project description:We have previously shown that conditional overexpression of Bmi1 in NSC increases their proliferation both in the developing neocortexand in the postnatal brain (Yadirgi et al. 2011). However, during embryonic development, increased and ectopic proliferation induced by overexpression of Bmi1 in progenitors committed toward a neuronal lineage triggered apoptosis, leading eventually to a reduced overall brain size (Yadirgi et al. 2011). These findings M-bM-^@M-^Sincreased proliferation of neural stem/progenitor cells (NSPC) and apoptosis of neuronal committed progenitors - could be faithfully reproduced in an in vitro assay where NSPC isolated from Nestin-Cre; STOP FloxBmi1 embryos are cultured in floating conditions in the presence of EGF and FGF2 (neurosphere assay). We isolated NSPC from Nestin-Cre;STOP FloxBmi1 E16.5 neocortices and cultured them briefly under neurosphere inducing conditions. We then analysed their transcriptome by whole-genome Illumina platform mouse v2 and compared it to the transcriptome of NSPC isolated from non-transgenic or single transgenic littermates. The objective of this analysis was to identify genes differentially expressed upon overexpression of the PcG gene Bmi1 in neural stem/progenitor cells. 4 samples, 2 condition with 2 biological replicates per condition

2012-11-12 | E-GEOD-31607 | biostudies-arrayexpress

Recurrent genomic instability of chromosome 1q in neural derivatives of human embryonic stem cells

Project description:array-CGH analysis of human neural stem cells derived from human ES cells during long term in vitro culture array-CGH analysis of human neural stem cells derived from human ES cells comparing control genomic DNA

2012-01-25 | E-GEOD-33708 | biostudies-arrayexpress

Genome-wide analysis of gene expression between a mouse genetic model of glioma (Mut6) and an origin cell type (neural stem cells)

Project description:Comparison of gene expression (mRNA) between neurosphere cultures from Mut6 gliomas and wt SVZ neural stem cells (NSCs) Total RNA obtained from neurosphere cultures from Mut6 gliomas was compared to those from wt SVZ

2014-12-08 | E-GEOD-34333 | biostudies-arrayexpress

Genome-wide analysis of gene expression between a mouse genetic model of glioma (Mut6) and an origin cell type (neural stem cells)

Project description:Comparison of gene expression (mRNA) between neurosphere cultures from Mut6 gliomas and wt SVZ neural stem cells (NSCs)

2014-12-08 | GSE34333 | GEO

Genes differentially expressed between Bmi1-overexpressing and empty vector control neural stem/progenitor cells

Project description:Neural stem/progenitor cells were isolated from the lateral ventricle wall of 4-6 week-old CD1 mice and grown as neurospheres under low density culture conditions. Test cells were transduced with bicistronic retroviral constructs for the over-expression of Bmi1 together with eGFP, and control cells were transduced with an empty vector construct expressing eGFP only. To identify genes, which are regulated by BMI1 in neural stem/progenitor cells, the gene expression profiles of neurosphere cells over-expressing Bmi1 were compared empty vector control cells using Affymetrix Gene mouse ST1.0 arrays 3 independent Bmi1-overexpressing neurosphere cultures (test samples) were compared to 3 independent empty vector neurosphere cultures (control samples).

2016-06-01 | E-GEOD-70451 | biostudies-arrayexpress

Transcription profiling of p53 knock-out mouse to analyse neural stem cell (neurosphere) prolifieration

Project description:The effects of gene p53 knock-out on mouse neural stem cell (neurosphere) prolifieration.

2005-11-15 | E-MEXP-483 | biostudies-arrayexpress

Differentially regulted genes in neural stem cells treated by oleanolic acid

Project description:Oleanolic acid significantly inhibited neurosphere formation in a dose-dependent manner, and achieved a maximum effect at 10 nM. OA also reduced the 5-ethynyl-2M-bM-^@M-^Y-deoxyuridine (EdU) incorporation into NSCs, indicating an inhibition on proliferation of NSCs. Next, in western blotting analysis, the protein expression of neuron-specific marker tubulin-M-NM-2M-bM-^EM-" (TuJ1) and Mash1 was increased, while the astrocyte-specific marker glial fibrillary acidic protein (GFAP) decreased. In immunofluorescence analysis, OA significantly elevated the percentage of TuJ1-positive cells and reduced GFAP-positive cells. Using DNA microarrays, 183 genes were found to be differentially regulated by OA. Neural stem cells derived form mouse embrynic brains were treated with Oleanolic acid or not. Each group had 4 biological relicates each from a mouse.

2012-06-01 | E-GEOD-38394 | biostudies-arrayexpress

Alternative routes to induced pluripotent stem cells revealed by reprogramming of neural lineage

Project description:During the reprogramming of mouse embryonic fibroblasts (MEFs) to induced pluripotent stem cells, the activation of pluripotency genes such as Nanog occurs after the mesenchymal to epithelial transition (MET). Here we report that both adult stem cells (neural stem cells- NSCs) and differentiated cells (astrocytes) of the neural lineage can activate Nanog in the absence of cadherin expression during reprogramming. Gene expression analysis revealed that only the Nanog+Ecadherin+ populations expressed stabilization markers and had upregulated several cell cycle genes; and were transgene independent. Inhibition of Dot1L activity, which has previously been shown to increase MET, enhanced both the numbers of Nanog+ and Nanog+E-cadherin+ colonies, suggesting a MET independent pathway for activation of Nanog in NSCs. Expressing Sox2 in MEFs prior to reprogramming does not alter the ratio of Nanog colonies that express E-cadherin obtained. Taken together these results provide a novel pathway for reprogramming taken by cells of the neural lineage. Neural Stem Cells (NSCs) were induced to reprogram by the induction of Oct4, Sox2, c-Myc and Klf4. Reprogramming colonies that expressed either Nanog alone (N+E-) or Nanog and E-cadherin (N+E+) were sorted by flow cytometry and used as input for RNA-sequencing. There are 3 replicates each for the N+E- and N+E+ samples.

2016-02-18 | E-GEOD-76130 | biostudies-arrayexpress

Direct reprogramming of human astrocytes into neural stem cells and neurons

Project description:Ectopic expression of the reprogramming factors OCT4, SOX2, or NANOG into human astrocytes in specific cytokine/culture conditions activated the neural stem gene program and induced generation of cells expressing neural stem/precursor markers. Here we compare the whole gene expression profile of primary human astrocytes (Astro) with neural stem cells (HNSC) derived from astrocytes reprogramming

2013-12-01 | E-GEOD-36145 | biostudies-arrayexpress

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