Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0

Microarray analysis of FoxP3-expressing or non-expressing subsets of human CD4+ T cells

ABSTRACT: Gene expression profiles of subsets of CD4+ T cells according to their expression of FoxP3 and CD45RA were compared. Abstract: FoxP3 is a key transcription factor for the development and function of natural CD4+ regulatory T cells (Tregs). Here we show that human FoxP3+CD4+ T cells are composed of three phenotypically and functionally distinct subpopulations: CD45RA+FoxP3low resting Tregs (rTregs) and CD45RA-FoxP3high activated Tregs (aTregs), both of which are suppressive in vitro, and cytokine-secreting CD45RA-FoxP3low non-suppressive T cells. The proportion of the three subpopulations characteristically altered in cord blood, aged individuals, and patients with immunological diseases. Terminally differentiated aTregs rapidly die while rTregs proliferate and convert into aTregs in vitro and in vivo as shown by the transfer of rTregs into NOD-scid-common gamma-chain-knockout mice and by TCR sequence-based T cell clonotype tracing in peripheral blood of normal individuals. Taken together, the dissection of FoxP3+ cells into subsets enables one to analyze Treg differentiation dynamics and interactions in normal and disease states, and to control immune responses through manipulating particular FoxP3+ subpopulations. RNA was extracted from freshly obtained peripheral blood lymphocytes from a healthy donor that were separated according to their expression of CD25, CD127 and CD45RA after surface staining.

ORGANISM(S): Homo sapiens

SUBMITTER: Makoto Miyara 

PROVIDER: E-GEOD-15659 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

Json Xml
altmetric image

Publications

FoxP3 is a key transcription factor for the development and function of natural CD4(+) regulatory T cells (Treg cells). Here we show that human FoxP3(+)CD4(+) T cells were composed of three phenotypically and functionally distinct subpopulations: CD45RA(+)FoxP3(lo) resting Treg cells (rTreg cells) and CD45RA(-)FoxP3(hi) activated Treg cells (aTreg cells), both of which were suppressive in vitro, and cytokine-secreting CD45RA(-)FoxP3(lo) nonsuppressive T cells. The proportion of the three subpopu  ...[more]

Similar Datasets

Unknown Microarray analysis of FoxP3-expressing or non-expressing subsets of human CD4+ T cells
2009-05-21 | GSE15659 | GEO
Transcriptomics Profiling of naïve and memory Tregs
2016-01-19 | E-GEOD-76598 | biostudies-arrayexpress
Unknown Profiling of naïve and memory Tregs
2016-01-19 | GSE76598 | GEO
Transcriptomics Gene expression profiling of CD4+CD25+Foxp3+ regulatory T cells and CD4+CD25-Foxp3- T cells in the course of Plasmodium yoelii infection of BALB/c mice
2012-04-15 | E-GEOD-34621 | biostudies-arrayexpress
Unknown Gene expression profiling of CD4+CD25+Foxp3+ regulatory T cells and CD4+CD25-Foxp3- T cells in the course of Plasmodium yoelii infection of BALB/c mice
2012-04-16 | GSE34621 | GEO
Transcriptomics Mbd2 promotes Foxp3 demethylation and T-regulatory cell function
2013-09-01 | E-GEOD-48653 | biostudies-arrayexpress
Transcriptomics Critical Requirement of the DNA Methyltransferase, Dnmt1, for the Development and Function of Foxp3+ Regulatory T Cells
2013-04-21 | E-GEOD-27434 | biostudies-arrayexpress
Unknown Gene expression profiling of effector and regulatory T-cells from peripheral blood of rheumatoid arthritis (RA) patients and healthy volunteers.
2015-09-05 | GSE65010 | GEO
Transcriptomics Transcription profiling of human fetal thymocytes and circulating naive T cells
2007-08-06 | E-GEOD-1460 | biostudies-arrayexpress
Proteomics CD49f defines a subset of dysfunctional regulatory T cells circulating in the human peripheral blood
2021-08-06 | PXD022095 | Pride