Human Mammary Epithelial Cell Response to EGF, microarray analysis

ABSTRACT: To understand how integration of multiple data types can help decipher cellular responses at the systems level, we analyzed the mitogenic response of human mammary epithelial cells to epidermal growth factor (EGF) using whole genome microarrays, mass spectrometry-based proteomics and large-scale western blots with over 1000 antibodies. A time course analysis revealed significant differences in the expression of 3172 genes and 596 proteins, including protein phosphorylation changes measured by western blot. Integration of these disparate data types showed that each contributed qualitatively different components to the observed cell response to EGF and that varying degrees of concordance in gene expression and protein abundance measurements could be linked to specific biological processes.

ORGANISM(S): Homo sapiens

SUBMITTER: Katrina Waters 

PROVIDER: E-GEOD-15668 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress