Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0

Expression data from batch cultivations with A. niger, A. oryzae and A. nidulans grown on glucose and glycerol

ABSTRACT: Conserved transcriptional regulation of glycerol metabolism was investigated in three Aspergillus species, A. nidulans, A. oryzae and A. niger. For this purpose, batch cultivations under well controlled conditions were performed with the three Aspergilli. Samples for RNA extraction were collected and further processed for hybridization in custom designed Affymetrix microarrays containing probes for the three Aspergillus species. Protein comparisons and cross analysis with gene expression data of all three species resulted in the identification of 88 genes having a conserved response across the three Aspergillus species. A promoter analysis of the up-regulated genes led to the identification of a conserved binding site for a putative regulator. Triplicate batch fermentations with each of the three Aspergillus species were carried out and transcriptome analysis was performed. Biomass from each Aspergillus species was harvested in the exponential phase of growth and further processed for RNA extraction and hybridization on Affymetrix microarrays.

ORGANISM(S): Aspergillus oryzae

SUBMITTER: Wanwipa Vongsangnak 

PROVIDER: E-GEOD-15702 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

Json Xml
altmetric image

Publications

Uncovering transcriptional regulation of glycerol metabolism in Aspergilli through genome-wide gene expression data analysis.

Salazar Margarita M   Vongsangnak Wanwipa W   Panagiotou Gianni G   Andersen Mikael R MR   Nielsen Jens J  

Molecular genetics and genomics : MGG 20090926 6

Glycerol is catabolized by a wide range of microorganisms including Aspergillus species. To identify the transcriptional regulation of glycerol metabolism in Aspergillus, we analyzed data from triplicate batch fermentations of three different Aspergilli (Aspergillus nidulans, Aspergillus oryzae and Aspergillus niger) with glucose and glycerol as carbon sources. Protein comparisons and cross-analysis with gene expression data of all three species resulted in the identification of 88 genes having  ...[more]

Similar Datasets

Proteomics Similar is not the same: Differences in the function of the (hemi-)cellulolytic regulator XlnR (Xlr1/Xyr1) in filamentous fungi
2014-08-01 | PXD001190 | Pride
Transcriptomics Expression data from batch cultivations of Aspergillus niger wild type and adrA, facB and creA deletion mutants
2011-01-31 | E-GEOD-19952 | biostudies-arrayexpress
Transcriptomics Expression data from batch cultivations with the acidogenic Aspergillus niger wild type strain ATCC 1015 and the areB complete deletion strain constructed using ATCC 1015 as background strain with glucose or glycerol as carbon sources.
2011-01-31 | E-GEOD-21015 | biostudies-arrayexpress
Proteomics Mixed colonies of Aspergillus niger and Aspergillus oryzae cooperatively degrading wheat bran
2017-02-27 | PXD005532 | Pride
Unknown Expression data from batch cultivations with A. niger, A. oryzae and A. nidulans grown on glucose and glycerol
2009-10-21 | GSE15702 | GEO
Transcriptomics Expression analysis of Aspergillus nidulans OE::rsmA
2012-01-17 | E-GEOD-34220 | biostudies-arrayexpress
Transcriptomics A p53-like like transcription factor similar to Ndt80 controls the response to nutrient stress in the filamentous fungus, Aspergillus nidulans
2013-01-31 | E-GEOD-36235 | biostudies-arrayexpress
Unknown A tri-species Aspergillus array (oryzae arrays)
2008-02-15 | GSE9277 | GEO
Unknown A tri-species Aspergillus array (niger arrays)
2008-02-15 | GSE9276 | GEO
Unknown A tri-species Aspergillus array (nidulans arrays)
2008-02-15 | GSE9275 | GEO