Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

AURATUS goldfish environmental genomics projects: sulpiride in the telencephalon

ABSTRACT: Sexually regressing female goldfish were administered 2 i.p. injections: 1) 6 ug/g sulpiride in 0.1% DMSO and 0.6% saline, and; 2) 0.6% saline Tissues were harvested 5h post-injection. A common reference design was used: 4 independent groups of telencephali from sexually regressing female goldfish treated with 6 ug/g sulpiride hybridized against a common vehicle control. Slides 12, 26, 37, 48

ORGANISM(S): Carassius auratus

SUBMITTER: Jason Popesku

PROVIDER: E-GEOD-15705 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Publications

Meta-type analysis of dopaminergic effects on gene expression in the neuroendocrine brain of female goldfish.

Popesku Jason T JT Martyniuk Christopher J CJ Trudeau Vance L VL

Frontiers in endocrinology 20121102

Dopamine (DA) is a major neurotransmitter important for neuroendocrine control and recent studies have described genomic signaling pathways activated and inhibited by DA agonists and antagonists in the goldfish brain. Here we perform a meta-type analysis using microarray datasets from experiments conducted with female goldfish to characterize the gene expression responses that underlie dopaminergic signaling. Sexually mature, pre-spawning [gonadosomatic index (GSI) = 4.5 ± 1.3%] or sexually regr ...[more]

PMID: 23130016

Similar Datasets

Project description:Sex pheromones rapidly affect endocrine physiology and behaviour, but little is known about their effects on gene expression in the neuroendocrine tissues that mediate olfactory processing. In this study we exposed male goldfish for 6 h to waterborne 17,20betaP (4.3 nM) and PGF2α (3 nM), the main pre-ovulatory and post-ovulatory pheromones, respectively. Both treatments elevated milt volume (P = 0.001). Microarray hybridizations were used to male telencephalon samples following PGF2α treatment and identified 71 unique transcripts that were differentially expressed (q < 5%; 67 up, 4 down). Functional annotation of these regulated genes indicates that PGF2α pheromone exposure affects diverse biological processes including nervous system functions, energy metabolism, cholesterol/lipoprotein transport, translational regulation, transcription and chromatin remodelling, protein processing, cytoskeletal organization, and signalling. By using real-time RT-PCR, we further validated three candidate genes, ependymin-II, calmodulin-A and aldolase C, which exhibited 3 - 5 fold increase in expression following PGF2α exposure. Expression levels of some other genes that are thought to be important for reproduction were also determined using real-time RT-PCR. Expression of sGnRH was increased by PGF2α, but not 17,20betaP, whereas cGnRH expression was increased by 17,20betaP but not PGF2α. In contrast, both pheromones increase the expression of glutamate (GluR2a, NR2A) and gamma-aminobutyric acid (GABAA gamma2) receptor subunit mRNAs. This gene expression data link milt release and rapid modulation of neuronal transcription as part of the response to female sex pheromones. A total of four microarray slides were hybridized to study the effects of PGF2α on gene expression in the telencephalon. Control samples (N = 4) were pooled to form our reference or technical replicates while treatment samples (N = 4), which formed our biological replicates, were ran separately. In addition, two dye swaps were performed for our biological samples for the treatments.

Dataset Information

AURATUS goldfish environmental genomics projects: sulpiride in the telencephalon

Publications

Meta-type analysis of dopaminergic effects on gene expression in the neuroendocrine brain of female goldfish.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure