Reduced expression of RhoE promotes ROCK-dependent invasion in transformed human fibroblasts

ABSTRACT: Cen3tel cells, obtained by telomerase immortalization of human fibroblasts, gradually underwent neoplastic transformation and became metastatic in immunocompromised mice. Neoplastic transformation was associated with a change in cell morphology (from fibroblastic to polygonal). Tumorigenic cells acquired a clear-cut membrane localization of adhesion molecules, a reorganization of the actin cytoskeleton, increased cell motility and invasiveness. In a 3-dimensional environment, tumorigenic cells showed a spherical morphology with cortical actin rings, suggesting a switch from a mesenchymal to an amoeboid ROCK-dependent movement. Accordingly, cell invasion decreased upon treatment with the ROCK inhibitor Y27632, but not with the matrix protease inhibitor Ro28-2653. The increased invasiveness

ORGANISM(S): Homo sapiens

SUBMITTER: giovanna chiorino 

PROVIDER: E-GEOD-15742 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress