Homogeneous monocytes and macrophages from hES cells following coculture-free differentiation in M-CSF and IL-3.

ABSTRACT: In the present study we aimed at producing homogeneous populations of monocytes and macrophages from human embryonic stem (hES) cells. Human embryonic stem cell lines KCL001, KCL002, and HUES-2 were differentiated into monocytes by coculture-free differentiation with two growth factors using a three-step method. The method involved embryoid body (EB) formation in hES media, directed differentiation with macrophage colony-stimulating factor and interleukin (IL)-3, and harvest of nonadherent monocytes from the culture supernatants. Transcriptome analysis of the esMC and the esMDM revealed a distinct myeloid signature that correlated with primary adult blood-derived monocytes and spleen tissue samples but not with other tissue samples tested. In conclusion we have developed a simple and eff

ORGANISM(S): Homo sapiens

SUBMITTER: Fernando Martinez Estrada 

PROVIDER: E-GEOD-15791 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress