Genome Variation in the Symbiotic Nitrogen-Fixing Bacterium Sinorhizobium meliloti

ABSTRACT: Differences in genome size and gene content are among the most important signatures of microbial adaptation and genome evolution. Here, we investigated the patterns of genome variation among strains of the symbiotic nitrogen-fixing bacterium Sinorhizobium meliloti. Using the sequenced strain Rm1021 as a reference, the genome size and gene content variations were analyzed among ten diverse natural strains, through pulse field gel electrophoresis (PFGE) and whole-genome microarray hybridizations. Our PFGE analysis showed a genome size range of 6.45-7.01Mbp, with the greatest variation arising from the pSymA replicon, followed by that of pSymB. No observable size difference was evident among the chromosomes. Consistent with this pattern of size differences, 41.2% of ORFs on pSymA were variabl

ORGANISM(S): Sinorhizobium meliloti

SUBMITTER: HONG GUO 

PROVIDER: E-GEOD-15889 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress