Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Gene expression pattern in CXCR4+ and CXCR4- subpopulation of breast cancer cells

ABSTRACT: The goal of this study was to identify signalling molecules downstream of CXCR4 in breast cancer cells. For this purpose, we sorted CXCR4-positive and CXCR4-negative cells from MDA-MB-231 breast cancer cell line by flow cytometry and performed microarrays analysis. Three sets of samples, each in quadruplicate, were analyzed. These include CXCR4-positive subpopulation, CXCR4-positive subpopulation treated with SDF-1 (ligand for CXCR4, also called CXCL12) for one hour and CXCR4-negative subpopulation.

ORGANISM(S): Homo sapiens

SUBMITTER: Harikrishna Nakshatri

PROVIDER: E-GEOD-15893 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

ITF2 is a target of CXCR4 in MDA-MB-231 breast cancer cells and is associated with reduced survival in estrogen receptor-negative breast cancer.

Appaiah Hitesh H Bhat-Nakshatri Poornima P Mehta Rutika R Thorat Mangesh M Badve Sunil S Nakshatri Harikrishna H

Cancer biology & therapy 20100904 6

CXCR4, a chemokine receptor, plays an important role in breast cancer growth, invasion, and metastasis. The transcriptional targets of CXCR4 signaling are not known. Microarray analysis of CXCR4-enriched and CXCR4-low subpopulations of the MDA-MB-231 breast cancer cell line, which has a constitutively active CXCR4 signaling network, revealed differential expression of ∼ 200 genes in the CXCR4-enriched subpopulation. ITF2, upregulated in CXCR4-enriched cells, was investigated further. Expression ...[more]

PMID: 20603605

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Dataset Information

Gene expression pattern in CXCR4+ and CXCR4- subpopulation of breast cancer cells

Publications

ITF2 is a target of CXCR4 in MDA-MB-231 breast cancer cells and is associated with reduced survival in estrogen receptor-negative breast cancer.

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