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Drosophila melanogaster small RNA sequence: Kc167 cells (Rubin lab)

ABSTRACT: modENCODE_submission_2374 This submission comes from a modENCODE project of Eric Lai. For full list of modENCODE projects, see http://www.genome.gov/26524648 Project Goal: We plan to generate a comprehensive catalog of expressed and functional microRNAs, and generate biological evidence for their regulatory activity. We plan also to delineate the primary transcription units of microRNA genes. Finally, we plan to annotate other classes of non-miRNA expressed small RNAs, as least some of which may define novel classes of small RNA genes. For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf EXPERIMENT TYPE: RNA-seq. BIOLOGICAL SOURCE: Cell Line: Kc-Rubin; Tissue: embryo-derived cell-line; Developmental Stage: late embryonic stage; Genotype: se/e; Sex: Female; EXPERIMENTAL FACTORS: Cell Line Kc-Rubin; Replicate type: biological

ORGANISM(S): Drosophila melanogaster

SUBMITTER: DCC modENCODE

PROVIDER: E-GEOD-15899 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Drosophila melanogaster small RNA sequence: S2-DRSC cells (Rubin lab)

Project description:modENCODE_submission_2725 This submission comes from a modENCODE project of Eric Lai. For full list of modENCODE projects, see http://www.genome.gov/26524648 Project Goal: We plan to generate a comprehensive catalog of expressed and functional microRNAs, and generate biological evidence for their regulatory activity. We plan also to delineate the primary transcription units of microRNA genes. Finally, we plan to annotate other classes of non-miRNA expressed small RNAs, as least some of which may define novel classes of small RNA genes. For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf EXPERIMENT TYPE: RNA-seq. BIOLOGICAL SOURCE: Cell Line: S2-DRSC; Tissue: embryo-derived cell-line; Developmental Stage: late embryonic stage; Sex: Male; EXPERIMENTAL FACTORS: Cell Line S2-DRSC (Rubin); Replicate type: biological

2012-08-08 | E-GEOD-40016 | biostudies-arrayexpress

Drosophila melanogaster small RNA sequence: cell lines and imaginal disc

Project description:High-throughput sequencing of Drosophila melanogaster small RNAs. total RNA, ~18-26nt RNAs isolated using PAGE, ligation to adapters requires 5' monophosphate and 3' OH Keywords: High-throughput solexa sequencing Small RNAs were sequenced from D. melanogaster S2 and KC cells. Raw sequences were clipped by 3' linker sequences recognition, and select clipped sequences longer than 18 nt

2008-04-30 | E-GEOD-10794 | biostudies-arrayexpress

Drosophila melanogaster small RNA sequence from 14 libraries

Project description:High-throughput sequencing of Drosophila melanogaster small RNAs. total RNA, ~18-26nt RNAs isolated using PAGE, ligation to adapters requires 5' monophosphate and 3' OH Small RNAs were sequenced from D. melanogaster heads (male and female), body (male and female), S2 and Kc cells and different stages of embryo. Raw sequences were clipped by 3' linker sequences recognition, and select clipped sequences longer than 18 nt

2008-06-01 | E-GEOD-11624 | biostudies-arrayexpress

Drosophila melanogaster small RNA sequence: female heads

Project description:modENCODE_submission_750 This submission comes from a modENCODE project of Eric Lai. For full list of modENCODE projects, see http://www.genome.gov/26524648 Project Goal: We plan to generate a comprehensive catalog of expressed and functional microRNAs, and generate biological evidence for their regulatory activity. We plan also to delineate the primary transcription units of microRNA genes. Finally, we plan to annotate other classes of non-miRNA expressed small RNAs, as least some of which may define novel classes of small RNA genes. For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf EXPERIMENT TYPE: RNA-seq. BIOLOGICAL SOURCE: Strain: Canton-S; Tissue: adult head tissue; Replicate type: biological; Developmental Stage: adult; Sex: female; EXPERIMENTAL FACTORS: Strain: Canton-S

2012-08-09 | E-GEOD-40038 | biostudies-arrayexpress

Drosophila melanogaster Canton-S small RNA sequence: Adult Female Body

Project description:modENCODE_submission_2555 This submission comes from a modENCODE project of Eric Lai. For full list of modENCODE projects, see http://www.genome.gov/26524648 Project Goal: We plan to generate a comprehensive catalog of expressed and functional microRNAs, and generate biological evidence for their regulatory activity. We plan also to delineate the primary transcription units of microRNA genes. Finally, we plan to annotate other classes of non-miRNA expressed small RNAs, as least some of which may define novel classes of small RNA genes. For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf EXPERIMENT TYPE: RNA-seq. BIOLOGICAL SOURCE: Developmental Stage: Adult female; Genotype: wild type; Sex: Female; EXPERIMENTAL FACTORS: Strain Canton S; Tissue Female body; Replicate type: biological;

2012-08-08 | E-GEOD-40015 | biostudies-arrayexpress

Drosophila melanogaster Canton-S small RNA sequence: Adult Male Head

Project description:modENCODE_submission_747 This submission comes from a modENCODE project of Eric Lai. For full list of modENCODE projects, see http://www.genome.gov/26524648 Project Goal: We plan to generate a comprehensive catalog of expressed and functional microRNAs, and generate biological evidence for their regulatory activity. We plan also to delineate the primary transcription units of microRNA genes. Finally, we plan to annotate other classes of non-miRNA expressed small RNAs, as least some of which may define novel classes of small RNA genes. For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf EXPERIMENT TYPE: RNA-seq. BIOLOGICAL SOURCE: Strain: Canton S; Tissue: Male body; Developmental Stage: Adult male; Genotype: wild type; Sex: Male; EXPERIMENTAL FACTORS: Strain Canton S; Tissue Male head; Developmental Stage Adult male; Replicate type: biological;

2012-08-09 | E-GEOD-40034 | biostudies-arrayexpress

Drosophila melanogaster 6-10hr embryo

Project description:modENCODE_submission_754 This submission comes from a modENCODE project of Eric Lai. For full list of modENCODE projects, see http://www.genome.gov/26524648 Project Goal: We plan to generate a comprehensive catalog of expressed and functional microRNAs, and generate biological evidence for their regulatory activity. We plan also to delineate the primary transcription units of microRNA genes. Finally, we plan to annotate other classes of non-miRNA expressed small RNAs, as least some of which may define novel classes of small RNA genes. For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf EXPERIMENT TYPE: RNA-seq. BIOLOGICAL SOURCE: Strain: Canton-S; Tissue: embryo; Replicate type: biological; Developmental Stage: embryonic 6-10hr; Sex: NA; EXPERIMENTAL FACTORS: Strain: Canton-S

2012-08-09 | E-GEOD-40040 | biostudies-arrayexpress

Drosophila melanogaster 0-1hr embryo

Project description:modENCODE_submission_755 This submission comes from a modENCODE project of Eric Lai. For full list of modENCODE projects, see http://www.genome.gov/26524648 Project Goal: We plan to generate a comprehensive catalog of expressed and functional microRNAs, and generate biological evidence for their regulatory activity. We plan also to delineate the primary transcription units of microRNA genes. Finally, we plan to annotate other classes of non-miRNA expressed small RNAs, as least some of which may define novel classes of small RNA genes. For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf EXPERIMENT TYPE: RNA-seq. BIOLOGICAL SOURCE: Strain: Canton-S; Tissue: embryo; Replicate type: biological; Developmental Stage: embryonic 0-1hr; Sex: NA; EXPERIMENTAL FACTORS: Strain: Canton-S

2012-08-09 | E-GEOD-40042 | biostudies-arrayexpress

Drosophila melanogaster 2-6hr embryo

Project description:modENCODE_submission_752 This submission comes from a modENCODE project of Eric Lai. For full list of modENCODE projects, see http://www.genome.gov/26524648 Project Goal: We plan to generate a comprehensive catalog of expressed and functional microRNAs, and generate biological evidence for their regulatory activity. We plan also to delineate the primary transcription units of microRNA genes. Finally, we plan to annotate other classes of non-miRNA expressed small RNAs, as least some of which may define novel classes of small RNA genes. For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf EXPERIMENT TYPE: RNA-seq. BIOLOGICAL SOURCE: Strain: Canton-S; Tissue: embryo; Replicate type: biological; Developmental Stage: embryonic 2-6hr; Sex: NA; EXPERIMENTAL FACTORS: Strain: Canton-S

2012-08-09 | E-GEOD-40039 | biostudies-arrayexpress

Drosophila melanogaster Pupae 2-4 days

Project description:modENCODE_submission_757 This submission comes from a modENCODE project of Eric Lai. For full list of modENCODE projects, see http://www.genome.gov/26524648 Project Goal: We plan to generate a comprehensive catalog of expressed and functional microRNAs, and generate biological evidence for their regulatory activity. We plan also to delineate the primary transcription units of microRNA genes. Finally, we plan to annotate other classes of non-miRNA expressed small RNAs, as least some of which may define novel classes of small RNA genes. For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf EXPERIMENT TYPE: RNA-seq. BIOLOGICAL SOURCE: Strain: Canton-S; Tissue: pupae; Replicate type: biological; Developmental Stage: Pupae 2-4d; Sex: NA; EXPERIMENTAL FACTORS: Strain: Canton-S

2012-08-09 | E-GEOD-40043 | biostudies-arrayexpress

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