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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Gene expression from bronchial and nasal epithelial cell samples of healthy current and never smokers.

ABSTRACT: mRNA expression was assayed from bronchial epithelial cells collected via bronchoscopy and nasal epithelial cells collected by brushing the inferior turbinate from healthy current and never smoker volunteers in order to determine the relationship between smoking-related gene expression changes in bronchial and nasal epithelium within the same individual. Bronchial epithelial cells were collected from current and never smokers via bronchoscopy, and nasal epithelial cells were collected by brushing the inferior turbinate during the same clinic visit. 1ug of RNA was isolated and hybridized to Affymetrix Human Exon 1.0 ST microarrays to obtain mRNA expression. The genome build upon which transcript assignments are based is hg18 (HuEx-1_0-st-v2.na27.hg18.transcript.csv).

ORGANISM(S): Homo sapiens

SUBMITTER: Xiaoling Zhang

PROVIDER: E-GEOD-16008 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Similarities and differences between smoking-related gene expression in nasal and bronchial epithelium.

Zhang Xiaoling X Sebastiani Paola P Liu Gang G Schembri Frank F Zhang Xiaohui X Dumas Yves Martine YM Langer Erika M EM Alekseyev Yuriy Y O'Connor George T GT Brooks Daniel R DR Lenburg Marc E ME Spira Avrum A

Physiological genomics 20091201 1

Previous studies have shown that physiological responses to cigarette smoke can be detected via bronchial airway epithelium gene expression profiling and that heterogeneity in this gene expression response to smoking is associated with lung cancer. In this study, we sought to determine the similarity of the effects of tobacco smoke throughout the respiratory tract by determining patterns of smoking-related gene expression in paired nasal and bronchial epithelial brushings collected from 14 healt ...[more]

PMID: 19952278

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