Loss of the Mili-interacting Tdrd1 activates transposons and alters Mili-associated small RNA profile

ABSTRACT: Piwi proteins and their associated Piwi-interacting RNAs (piRNAs) are implicated in transposon silencing in the murine germline. There is currently little information on additional proteins in the murine Piwi complex and how they might regulate the entry of transcripts that accumulate as piRNAs in the Piwi ribonucleoprotein (piRNP). We isolated Mili-containing complexes from adult mouse testes and identified Tudor domain-containing protein 1 (Tdrd1) as a factor specifically associated with the Mili piRNP throughout spermatogenesis. Complex formation is promoted by the recognition of symmetrically dimethylated arginines at the N-terminus of Mili by the Tudor domains of Tdrd1. Similar to a Mili mutant, mice lacking Tdrd1 show derepression of L1 transposons accompanied by a loss of DNA methyl

ORGANISM(S): Mus musculus

SUBMITTER: Ramesh Pillai 

PROVIDER: E-GEOD-16023 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress