Distinctions between the stasis and telomere attrition senescence barriers in cultured human mammary epithelial cells

ABSTRACT: Molecular distinctions between the stasis and telomere attrition senescence barriers in cultured human mammary epithelial cells Normal human epithelial cells in culture have generally shown a limited proliferative potential of ~10-40 population doublings before encountering a stress-associated senescence barrier (stasis) associated with elevated levels of cyclin-dependent kinase inhibitors p16 and/or p21. We now show that simple changes in media composition can expand the proliferative potential of human mammary epithelial cells (HMEC) initiated as primary cultures to 50-60 population doublings, followed by p16(+), senescence-associated b-galactosidase(+) stasis. We compared the properties of growing and senescent pre-stasis HMEC with growing and senescent post-selection HMEC, i.e., cells

ORGANISM(S): Homo sapiens

SUBMITTER: Sanchita Bhattacharya 

PROVIDER: E-GEOD-16058 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress