Generation of Induced Pluripotent Stem Cells using Recombinant Proteins

ABSTRACT: Groundbreaking work demonstrated that ectopic expression of four transcription factors, Oct4, Klf4, Sox2, and c-Myc, could reprogram murine somatic cells to induced pluripotent stem cells (iPSCs) (Takahashi and Yamanaka, 2006), and human iPSCs were subsequently generated using similar genetic manipulation (Takahashi et al., 2007,Yu et al., 2007). To address the safety issues arose from harboring integrated exogenous sequences in the target cell genome, a number of modified genetic methods have been developed and produced iPSCs with potentially reduced risks (for discussion, see Yamanaka, 2009, and references therein). However, all of the methods developed to date still involve the use of genetic materials and thus the potential for unexpected genetic modifications by the exogenous sequence

ORGANISM(S): Mus musculus

SUBMITTER: T Lin 

PROVIDER: E-GEOD-16062 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress