The Transcriptionally Active Regions in the Genome of Bacillus subtilis

ABSTRACT: The majority of all genes have so far been identified and annotated systematically through in silico gene finding. Here we report the finding of 3,217 strand-specific Transcriptionally Active Regions (TARs) in the genome of Bacillus subtilis by the use of tiling arrays. We have measured the genome-wide expression during mid-exponential growth on rich (LB) and minimal (M9) medium. The identified TARs account for 81.5% of the genes as they are currently annotated and additionally we find 44 novel protein-coding genes, 85 putative non-coding RNAs (ncRNAs) and 184 antisense transcripts. Hybridization of labeled genomic DNA (gDNA) has revealed a few thousand regions giving rise to very low signals. These are mostly caused by sequence errors, as is observed in the highly degenerate trp operon

ORGANISM(S): Bacillus subtilis subsp. subtilis str. 168

SUBMITTER: Simon Rasmussen 

PROVIDER: E-GEOD-16086 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress