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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of parental lines and bulked salt sensitive and salt tolerant RILs derived from 2 rice varieties

ABSTRACT: The aim of this study was to minimize the number of candidate genes responsible for salt tolerance between a pair of rice varieties (CSR27 and MI48) with contrasting level of salt tolerance by bulked segregant analysis of their recombinant inbred lines. Microarray analysis of RNA extracted from the tolerant and susceptible parents without and with stress showed 798 and 2407 differentially expressed genes, respectively. The number of differentially expressed genes was drastically reduced to 70 and 30, by pooling the RNAs from ten extreme tolerant and ten extreme susceptible RILs due to normalization of irrelevant differentially expressed genes between the parents. RNA from CSR27, MI48, tolerant bulk and susceptible bulk grown under control and salt stress conditions were analysed in two different biological replications (A and B) making total sixteen samples

ORGANISM(S): Oryza sativa Indica Group

SUBMITTER: Subhasisa Bal

PROVIDER: E-GEOD-16108 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Transcription profiling of parental lines and bulked salt sensitive and salt tolerant RILs derived from 2 rice varieties

Project description:The aim of this study was to minimize the number of candidate genes responsible for salt tolerance between a pair of rice varieties (CSR27 and MI48) with contrasting level of salt tolerance by bulked segregant analysis of their recombinant inbred lines. Microarray analysis of RNA extracted from the tolerant and susceptible parents without and with stress showed 798 and 2407 differentially expressed genes, respectively. The number of differentially expressed genes was drastically reduced to 70 and 30, by pooling the RNAs from ten extreme tolerant and ten extreme susceptible RILs due to normalization of irrelevant differentially expressed genes between the parents.

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Transcription profiling of indica rice cultivar FR13A under salt stress condition.

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Transcriptional profiling of indica rice cultivar FR13A under salt stress condition.

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microRNA expression in salt-tolerant and salt-sensitive cotton cultivars under normal and salt-stressed conditions

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Transcription profiling of two highly salt-tolerant wheat lines, their parental lines, and a salt-sensitive line in salt stress and control growth conditions

Project description:Comparative expression analysis between two highly salt-tolerant wheat lines, their parental lines, and a salt-sensitive line. The Chinese Spring (CS) line is salt sensitive. AJDAj5 and PhI are the two parental lines (with Chinese Spring background), and both have some salt-tolerance. W4909 and W4910 are two lines derived from crossing AJDAj5 and PhI, and both are more salt tolerant than either parental line.

2007-07-26 | E-MEXP-971 | biostudies-arrayexpress

microRNA expression in salt-tolerant and salt-sensitive cotton cultivars under normal and salt-stressed conditions

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Identification of non-coding RNA in sweet sorghum under salt stress

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Analysis of the transcriptomes of salt tolerant and susceptible cultivars of indica rice by microarray

Project description:Over the years, many traditional rice varietites of India were sourced and studied owing to their ability to withstand abiotic pressures like excessive salinity in the soil and water. These cultivars growing in specific areas of the country represent a rich gene pool from where a deeper understanding of the processes underlying tolerance to abiotic stress can be gained. Indigenous varieties like Nonabokra and Pokkali are known salt tolerant varieties and are being studied in great detail. In the present study, we have analyzed the transcriptomes of the contrasting cultivars; Nonabokra (tolerant), Pokkali (tolerant) and IR29 (susceptible) in order to decipher the differences in their responses to salinity stress by utilizing microarray.

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Gene expression profile for SvGRP-transformant and WT Arabidopsis seedlings under salt stress

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