Transcription profiling of mouse 26-day-old K-ras conditional mutant mice and 3-month-old K-ras conditional mutant mice reveals cell-specific Kras and Pten mutations document proliferation arrest in granulosa cells vs. oncogenic insult to OSE cells

ABSTRACT: The small G-protein KRAS is crucial for mediating gonadotropin-induced events associated with ovulation. However, constitutive expression of KrasG12D in granulosa cells disrupted normal follicle development leading to the persistence of abnormal follicle-like structures containing non-mitotic cells. To determine what factors mediate this potent effect of KrasG12D, gene profiling analyses were done. We also analyzed KrasG12D;Cyp19-Cre and KrasG12D;Pgr-Cre mutant mouse models that express Cre prior to or after the initiation of granulosa cell differentiation, respectively. KrasG12D induced cell cycle arrest in granulosa cells of the KrasG12D;Cyp19-Cre mice but not in the KrasG12D;Pgr-Cre mice, documenting the cell context specific effect of KrasG12D. Expression of KrasG12D silenced the Kras

ORGANISM(S): Mus musculus

SUBMITTER: Zhilin Liu 

PROVIDER: E-GEOD-16114 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress