454 Life Sciences/Roche and Illumina sequencing of small RNAs from planarian samples

ABSTRACT: Freshwater planarian flatworms possess uncanny regenerative capacities mediated by abundant and collectively totipotent adult stem cells. Key functions of these cells during regeneration and tissue homeostasis have been shown to depend on PIWI, a molecule required for piRNA expression in planarians. Nevertheless, the full complement of piRNAs, and microRNAs (miRNAs) in this organism has yet to be defined. Here, we report on the large scale cloning and sequencing of small RNAs from the planarian Schmidtea mediterranea, yielding altogether millions of sequenced unique small RNAs. We show that piRNAs are in part organized in genomic clusters and that they share characteristic features with mammalian and fly piRNAs. We further identify 61 novel miRNA genes and thus double the number of known p

ORGANISM(S): Schmidtea mediterranea

SUBMITTER: Marc Friedlaender 

PROVIDER: E-GEOD-16159 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress