Novel microRNAs uncovered by deep sequencing of small RNA transcriptomes in T. aestivum and B. distachyon

ABSTRACT: The small RNA transcriptomes of bread wheat (Triticum aestivum L.) and its emerging model (Brachypodium distachyon (L.) Beauv) were obtained by using deep sequencing technology. Small RNA compositions were analyzed in these two species. In addition to 70 conserved microRNAs (miRNA) from 25 families, 23 novel wheat miRNAs were identified. For Brachypodium, 12 putative miRNAs were predicted from a limited number of ESTs, of which one was a potential novel miRNA. Also, 94 conserved miRNAs from 28 families were identified in this species. Expression validation was performed for several novel wheat miRNAs. RNA ligase-mediated 5' RACE experiments demonstrated their capability to cleave predicted target genes including three disease resistant gene analogs. Differential expression of miRNAs was ob

ORGANISM(S): Triticum aestivum

SUBMITTER: bo wei 

PROVIDER: E-GEOD-16177 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress