Interfering with Proximal TLR4 to NF-kappaB Signal Transduction in Human Monocytes

ABSTRACT: This study investigated the global effect of carbon monoxide (CO) gas (250 ppm) on LPS- induced gene expression in THP-1 cells. CO predominantly suppresses LPS-induced gene response. Of the CO-suppressed genes, 18% were transcription factors and most others were cytokines, chemokines and immune response genes. Sequence analysis B binding siteskappafound that 81% of the gene promoters have putative NF- These results suggest that CO inhibits LPS-induced inflammatory B signaling pathwayresponse through regulating NF- The microarrays were performed on THP-1 cells, a human monocytic cell line. Cells were treated with or without LPS (1 ug/ml) in presence or absence of carbon monoxide (250 ppm) for 1 h. Total RNA was isolated, reverse transcribed, labeled and hybridized to oligonucleotide microarrays.

ORGANISM(S): Homo sapiens

SUBMITTER: Peter Munson 

PROVIDER: E-GEOD-16193 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress