Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Profiling gene expression in HeLa cells following TFEB transient transfection

ABSTRACT: Total RNA samples from three biological replicates in which TFEB was transiently overexpressed in HeLa cells by transfection using a pcDNA3 vector. As negative control, we used total RNA samples from HeLa cells transfected with an empty pcDNA3 vector. TFEB transfection

ORGANISM(S): Homo sapiens

SUBMITTER: Andrea Ballabio 

PROVIDER: E-GEOD-16267 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Lysosomes are organelles central to degradation and recycling processes in animal cells. Whether lysosomal activity is coordinated to respond to cellular needs remains unclear. We found that most lysosomal genes exhibit coordinated transcriptional behavior and are regulated by the transcription factor EB (TFEB). Under aberrant lysosomal storage conditions, TFEB translocated from the cytoplasm to the nucleus, resulting in the activation of its target genes. TFEB overexpression in cultured cells i  ...[more]