Elucidating Host-Pathogen Interaction in Acute Systemic Candidiasis in a Mouse Model Using Genomic Approach

ABSTRACT: Candida species is a common fungus that had evolved as both commensal and opportunistic pathogen in humans. The rise in human morbidity and mortality caused by Candida species sparked an alarming concern. However, the thin line between commensalism and infection as well as the analysis of Candida host-pathogen interactions has not been completely elucidated prior to the birth of global gene expression technologies. Gene expression analysis of host response upon systemic candidiasis had been carried out using Beadarray and validated by using GenomeLab™ GeXP multiplex PCR. Whole blood from infected mouse was isolated, globin mRNA depleted through subtractive hybridization and its RNA purified. Results from microarray data demonstrated increased expression of genes involved in the pathogen r

ORGANISM(S): Mus musculus

SUBMITTER: Phelim Yong 

PROVIDER: E-GEOD-16339 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress