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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of lymphatic endothelial cells infected with Kaposi's sacoma associated herpes virus

ABSTRACT: This SuperSeries is composed of the following subset Series: GSE16353: The profile of cellular and KSHV microRNAs in AIDS_KS biopsies (and normal skin control biopsies) GSE16354: Infection of Lymphatic and Blood Vessel Endothelial Cells (LEC and BEC) with KSHV GSE16355: Lymphatic endothelial cells (LEC) transfected with the KSHV microRNA cluster GSE16356: Lymphatic endothelial cells (LEC) treated with a MAF-targeted siRNA Refer to individual Series

ORGANISM(S): Homo sapiens

SUBMITTER: Stephen Henderson

PROVIDER: E-GEOD-16357 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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KSHV-encoded miRNAs target MAF to induce endothelial cell reprogramming.

Hansen Amy A Henderson Stephen S Lagos Dimitrios D Nikitenko Leonid L Coulter Eve E Roberts Sinead S Gratrix Fiona F Plaisance Karlie K Renne Rolf R Bower Mark M Kellam Paul P Boshoff Chris C

Genes & development 20100101 2

Kaposi sarcoma herpesvirus (KSHV) induces transcriptional reprogramming of endothelial cells. In particular, KSHV-infected lymphatic endothelial cells (LECs) show an up-regulation of genes associated with blood vessel endothelial cells (BECs). Consequently, KSHV-infected tumor cells in Kaposi sarcoma are poorly differentiated endothelial cells, expressing markers of both LECs and BECs. MicroRNAs (miRNAs) are short noncoding RNA molecules that act post-transcriptionally to negatively regulate gen ...[more]

PMID: 20080955

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Transcription profiling of human lymphatic edothelial cells transfected with the KSHV microRNA cluster

Project description:Kaposi sarcoma is the most common cancer in AIDS patients and is typified by red skin lesions. The disease is caused by the KSHV virus (HHV8) and is recognisable by its distinctive red skin lesions. The lesions are KSHV-infected spindle cells, most commonly the lymphatic endothelial and blood vessel endothelial cells (LEC and BEC), plus surrounding stroma. The KSHV virus expresses multiple microRNA in a single cluster. Here we test the effects of this KSHV microRNA cluster in LEC cells using Affymetrix hgu133plus2 chips. Experiment Overall Design: There are n=3 of: 1. LEC control with empty vector pSIN-MCS (LEC), 2. LEC transfected with the same vector containing the KSHV microRNA cluster.

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Transcription profiling of human LEC cells reveals KSHV manipulates notch signaling by upregulating Dll4 and JAG1 to alter cell cycle gene expression in

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Label free quantitative (LFQ) proteomic analysis of mouse lymphatic endothelial cells and primary mouse dendritic cells in response to IFNgamma treatment in presence or absence of 3-HKA

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2011-05-05 | E-GEOD-26341 | biostudies-arrayexpress

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