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Limitations and possibilities of small RNA digital gene expression profiling: library preparation comparison (454)

ABSTRACT: Analysis of RNA samples by massive parallel sequencing holds the promise to assay gene expression in both a quantitative and qualitative fashion and therefore allows for digital gene expression (DGE) profiling. We assessed the effect of different experimental approaches by generating small RNA libraries from a biological sample as well as an equimolar pool of synthetic miRNAs and analyzed the results using capillary dideoxy sequencing and next-generation sequencing platforms (Roche/454, AB/SOLiD and Illumina/Solexa). Whereas different sequencing platforms provided highly similar results, large differences in DGE profiles were observed depending on the library preparation method used. Nevertheless, our results indicate that the preferential nature of the library preparation methods is systematic and highly reproducible and we show that DGE is well suited for the quantification of relative expression differences between samples. Keywords: Transcriptome analysis Examination of three different library preparation methods for small RNAs, two replicates per library method

ORGANISM(S): Rattus norvegicus

SUBMITTER: Elzo de Wit

PROVIDER: E-GEOD-16369 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Limitations and possibilities of small RNA digital gene expression profiling.

Linsen Sam E V SE de Wit Elzo E Janssens Georges G Heater Sheila S Chapman Laura L Parkin Rachael K RK Fritz Brian B Wyman Stacia K SK de Bruijn Ewart E Voest Emile E EE Kuersten Scott S Tewari Muneesh M Cuppen Edwin E

Nature methods 20090701 7

PMID: 19564845

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