Transcription profiling of poplar WRKY23 reveals a regulatory role in defense: WRKY23-RNAi

ABSTRACT: To investigate the function of poplar WRKY23, we generated PtWRKY23-overexpressing and -underexpressing (RNAi) plants. Transgenic plants were inoculated with Melampsora rust or mock-inoculated for assessment of rust-resistance and for gene expression profiling using the poplar Affymetrix GeneChip® to study the consequences of PtWRKY23 overexpression and underexpression. Transcriptome analysis of PtWRKY23 overexpressors revealed a significant overlap with the Melampsora-infection response. Transcriptome analysis also indicated that PtWRKY23 affects redox homeostasis and cell wall-related metabolism. Experiment Overall Design: Leaf discs from wildtype, WRKY23-overexpressor and WRKY23-RNAi plants were inoculated with Melampsora medusae f. sp. tremuloidae (Mmt) or were mock-inoculated (contro

ORGANISM(S): Populus tremula x Populus alba

SUBMITTER: Armand Séguin 

PROVIDER: E-GEOD-16419 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress