Gene expression profile in CD4+ and CD8+ T cells from identical twins discordant for Multiple sclerosis

ABSTRACT: To gain insight into the etiopathogenesis of Multiple sclerosis (MS) we investigated gene expression changes in CD4+ and CD8+ T lymphocytes from monozygotic twins (MZ) discordant for relapsing remitting MS. We studied 4 monozygotic twin pairs discordant for disease, with the affected co-twin free of disease modifying therapies (F/M = 3/1, mean age 36.25±3.9). Following leukapheresis, CD4+ and CD8+ T cells were separated and studied by Affymetrix GeneChip® For gene expression profiling, peripheral blood mononuclear cell (PBMC) were obtained from leukopheresis by density centrifugation over Ficoll-hypaque according to standard procedures. Briefly, heparinized blood was diluted with 1 volume of RPMI medium and gently layered over the Fycoll. Following centrifugation at 660 g for 30 minutes, c

ORGANISM(S): Homo sapiens

SUBMITTER: Viviana Annibali 

PROVIDER: E-GEOD-16461 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress