Transcriptome analysis of rice (Oryza sativa) infected with blast fungus (Magnaporthe oryzae)
Ontology highlight
ABSTRACT: Rice blast is one of the most serious diseases and is caused by Magnaporthe grisea. SHZ-2, an indica cultivar with broad spectrum resistance to multiple races of the blast pathogen, was crossed to TXZ-13, a blast susceptible but high-quality variety, to produce one BC3 line, BC10 line, which showed strong to moderate blast resistance over eight cropping seasons in the field. In this study, we compared the transcription between blast-resistant and -susceptive lines by custom microarray. Keywords: time course, blast infection, disease response Comparison between blast- and mock-infected rice. Biological replicates: 3 control (mock) and 3 blast-infected at each time point, independently grown and harvested at 24 and 48 hours after inoculation. Each sample was prepared with whole plant 5 cm above ground and was pooled from 5 plants grown under the same conditions.
Project description:Rice tungro bacilliform virus (RTBV) is determinant of the rice tungro disease symptom. We compare the gene responses by RTBV infection between RTBV susceptive (TN1) and resistant cultivar (TW16). TN1 infected with RTBV shows the mild stunting and leaf yellowing, although TW16 shows only mild stunting at only early infection stage, and recovers the symptoms. Keywords: virus infection, disease response Comparison between RTBV and mock infected rice. Biological replicates: 3 control, 3 infected, independently grown and harvested. 1 samples derived from 5 plants grown under same conditons
Project description:This SuperSeries is composed of the following subset Series: GSE16140: Transcriptome analysis of rice (Oryza sativa cv.TW16) in relation to infection with rice tungro spherical virus (RTSV) GSE16141: Transcriptome analysis of rice (Oryza sativa cv. Taichung Native 1) in relation to infection with RTSV Refer to individual Series
Project description:Rice tungro disease is caused by the interaction between Rice tungro spherical virus (RTSV) and Rice tungro bacilliform virus. Infection with RTSV alone does not result in any distinctive symptoms in Taichung Native 1 (TN1) that is one of RTSV susceptive indica rice cultivar. To elucidate the basis of asymptomatic response of rice to RTSV at the gene expression level, global gene response in RTSV-infected TN1 was detected by custom microarray. Keywords: time course, virus infection, disease response Comparison between RTSV and mock infected rice. Biological replicates: 3 control (mock), 3 RTSV-infected at each time point, independently grown and harvested at 6 to 15 days after inoculation (DAI). 1 samples prepared with whole plant 5 cm above ground and pooled from 5 plants grown under same conditons.
Project description:Rice tungro disease is caused by the interaction between Rice tungro spherical virus (RTSV) and Rice tungro bacilliform virus. Infection with RTSV alone does not result in any distinctive symptoms in TW16 that is one of RTSV resistant indica rice. To elucidate the basis of asymptomatic response of rice to RTSV at the gene expression level, global gene response in RTSV-infected TN1 was detected by custom microarray. Keywords: time course, virus infection, disease response Comparison between RTSV and mock infected rice. Biological replicates: 3 control (mock), 3 RTSV-infected at each time point, independently grown and harvested at 6 to 15 days after inoculation (DAI). 1 samples prepared with whole plant 5 cm above ground and pooled from 5 plants grown under same conditons.
Project description:Near-isogenic lines TW16-4 and TW16-69 were developed from the cross between TN1 (recurrent parent susceptible to RTSV) and Utri Merah (donor parent resistant to RTSV). TW16-4 is susceptible to RTSV, whereas TW16-69 is resistant to RTSV based on a serolog. Experiment Overall Design: Comparison between RTSV and mock-infected rice. Biological replicates: 2 control (mock), 2 RTSV-infected, independently grown and harvested at 7 days after inoculation (DAI). Each sample was prepared using leaf tissues pooled from 5 plants grown under the same conditions.
Project description:Fourth leaves of rice seedlings (4.5 leaf stage) grown in hydroponic culture were inoculated with rice blast fungus and gene expression profiles were analyzed by microarray. Fourth leaves of the two isogenic lines of rice cv Nipponbare (blast-resistance gene: Pia or its mutant, pia) were inoculated with rice blast fungus, P91-15B, carrying avirulence gene, AvrPia. Total RNA was isolated, labeled with cy3, and probed with agilent rice oligoarray (4x44).
Project description:Fourth leaves of rice seedlings (4.5 leaf stage) grown in hydroponic culture were inoculated with rice blast fungus and gene expression profiles were analyzed by microarray. Fourth leaves of the two isogenic lines of rice cv Nipponbare (blast-resistance gene: Pish or its mutant, pish) were inoculated with rice blast fungus, Kyu77-07A, carrying avirulence gene, AvrPish. Total RNA was isolated, labeled with cy3, and probed with agilent rice oligoarray (4x44).
Project description:Rice grassy stunt disease is mainly observed in South, Southeast and East Asia, and caused by Rice grassy stunt virus (RGSV). Specific symptom appeared on RGSV infected rice plant is excess tillering. To guess how to induce excess tillering in RGSV infected plant, we analyzed the global gene expression changes in RGSV infected plants. Keywords: virus infection, disease response Comparison between RGSV and mock infected rice. Biological replicates: 3 control, 3 infected, independently grown and harvested. 1samples derived from 5 plants grown under same conditons
Project description:Rice gall dwarf virus (RGDV) is the causal agent of rice gall dwarf disease which causes severe loss of rice yield in Asia countries. In this study, we have analyzed the relationship between symptom and host gene responses by RGDV infection. Comparison between RGDV and mock infected rice. Biological replicates: 3 control, 3 infected, independently grown and harvested. 1 samples derived from 5 plants grown under same conditons
Project description:Rice ragged stunt virus (RRSV) is the causal agent of rice ragged stunt disease which causes severe loss of rice yield in Asia countries. In this study, we have analyzed the relationship between symptom and host gene responses by RRSV infection. Comparison between RRSV and mock infected rice. Biological replicates: 3 control, 3 infected, independently grown and harvested. 1 samples derived from 5 plants grown under same conditons